Инд. авторы:	Malsagova K.A., Pleshakova T.O., Popov V.P., Kupriyanov I.N., Galiullin R.A., Kozlov A.F., Shumov I.D., Kaysheva A.L., Tikhonenko F.V., Archakov A.I., Ivanov Y.D.
Заглавие:	Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
Библ. ссылка:	Malsagova K.A., Pleshakova T.O., Popov V.P., Kupriyanov I.N., Galiullin R.A., Kozlov A.F., Shumov I.D., Kaysheva A.L., Tikhonenko F.V., Archakov A.I., Ivanov Y.D. Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides // Micromachines. - 2021. - Vol.12. - Iss. 2. - Art.147. - ISSN 2072-666X.
Внешние системы:	DOI: 10.3390/mi12020147; PubMed: 33546438; WoS: 000622804400001;
Реферат:	eng: Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (similar to 10(-17) M) concentrations.
Ключевые слова:	DNA oligonucleotides; sensor chip; silicon nanoribbon; microRNA; autism spectrum disorders; Raman spectroscopy;
Издано:	2021
Физ. характеристика:	147