Инд. авторы:	Belousova I., Ershov N., Pavlushin S., Ilinsky Y., Martemyanov V.
Заглавие:	Molecular sexing of Lepidoptera
Библ. ссылка:	Belousova I., Ershov N., Pavlushin S., Ilinsky Y., Martemyanov V. Molecular sexing of Lepidoptera // Journal of Insect Physiology. - 2019. - Vol.114. - P.53-56. - ISSN 0022-1910. - EISSN 1879-1611.
Внешние системы:	DOI: 10.1016/j.jinsphys.2019.02.005; PubMed: 30776424; WoS: 000466618800008;
Реферат:	eng: We developed a universal method of Lepidoptera molecular sexing. The method is based on comparing the number of copies of the same gene in different sexes. Males of the majority of lepidopteran species have two Z chromosomes, whereas females have only one Z chromosome. Correspondingly, the number of copies of each gene located on this chromosome differs by two times between males and females. For quantitative estimation, we used qPCR. Via multiple alignment of the kettin (a Z chromosome gene) nucleotide sequences, we detected the most conserved fragment and designed primers with broad interspecies specificity for Lepidoptera. Using these primers, we successfully determined the sex of three lepidopteran species belonging to different superfamilies. The developed method is a simple, cost-effective and high-throughput technique for routine sexing. The sex of lepidopteran individuals can be examined at any developmental stage.
Ключевые слова:	MANDARINA; GENE; W-CHROMOSOME; BOMBYX-MORI; TRANSGENE COPY NUMBER; TIME QUANTITATIVE PCR; SILKWORM; Galleria mellonella; Plutella xylostella; Z chromosome; Kettin; Sex; Lymantria dispar; CHROMATIN;
Издано:	2019
Физ. характеристика:	с.53-56