Инд. авторы: Puzyr A.P., Medvedeva S.E., Burov A.E., Zernov Y.P., Bondar V.S.
Заглавие: Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis
Библ. ссылка: Puzyr A.P., Medvedeva S.E., Burov A.E., Zernov Y.P., Bondar V.S. Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis // Doklady Biochemistry and Biophysics. - 2018. - Vol.480. - Iss. 1. - P.173-176. - ISSN 1607-6729. - EISSN 1608-3091.
Внешние системы: DOI: 10.1134/S1607672918030146; РИНЦ: 35767153; PubMed: 30008104; SCOPUS: 2-s2.0-85050077028; WoS: 000438781500013;
Реферат: eng: In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 x 10(-5) - 1.4 x 10(-2) mu M was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 x 10(-11) g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.
Ключевые слова: MUSHROOM; ANTIOXIDANT;
Издано: 2018
Физ. характеристика: с.173-176
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