Инд. авторы:	Grizanova E.V., Semenova A.D., Komarov D.A., Chertkova E.A., Slepneva I.A., Dubovskiy I.M.
Заглавие:	Maintenance of redox balance by antioxidants in hemolymph of the greater wax moth Galleria mellonella larvae during encapsulation response
Библ. ссылка:	Grizanova E.V., Semenova A.D., Komarov D.A., Chertkova E.A., Slepneva I.A., Dubovskiy I.M. Maintenance of redox balance by antioxidants in hemolymph of the greater wax moth Galleria mellonella larvae during encapsulation response // Archives of Insect Biochemistry and Physiology. - 2018. - Vol.98. - Iss. 4. - Art.e21460. - ISSN 0739-4462. - EISSN 1520-6327.
Внешние системы:	DOI: 10.1002/arch.21460; PubMed: 29570844; WoS: 000438021300002;
Реферат:	eng: The lipid peroxidation process in hemocytes. activities of phenoloxidase and key enzymatic antioxidants (superoxide dismutase, glutathione-S-transferase, catalase) and nonenzymatic antioxidants (thiols, ascorbate) in hemolymph of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) were studied during the encapsulation process of nylon implants. It has been established that as soon as 15 min after piercing a cuticle with the implant, a capsule is formed on its surface. Active melanization of the capsule has been shown to last for 4 h. During the first hours after incorporating the implant, an increase in phenoloxidase activity and lipid peroxidation in the insect hemocytes has been revealed. Adhesion and degranulation on the surface of foreign object lead to the depletion of total hemocytes count (THC). Our results indicated that thiols and ascorbate molecules take part in the immediate antioxidant response, during later stages of encapsulation process hemolymph glutathione-Stransferase detoxifies and protects insect organism thereby restoring the internal redox balance. We suggest that nonenzymatic and enzymatic antioxidants of hemolymph plasma play a key role in the maintenance of redox balance during encapsulation of foreign targets.
Ключевые слова:	IMMUNITY; ASCORBIC-ACID; INSECT HEMOCYTES; DROSOPHILA-MELANOGASTER; MELANOTIC ENCAPSULATION; MANDUCA-SEXTA; OXIDATIVE STRESS; GLUTATHIONE-S-TRANSFERASES; GENERATION; ROS; redox balance; melanization; MDA; insect immunity; hemocytes; cellular immune response; antioxidant system; wax moth; PROPHENOLOXIDASE;
Издано:	2018