Инд. авторы: Simonov E.P., Wink M.
Заглавие: THE USE OF BUCCAL SWABS FOR DNA SAMPLING OF SCALED REPTILES (SQUAMATA): A COMPARATIVE ANALYSIS OF METHODS
Библ. ссылка: Simonov E.P., Wink M. THE USE OF BUCCAL SWABS FOR DNA SAMPLING OF SCALED REPTILES (SQUAMATA): A COMPARATIVE ANALYSIS OF METHODS // Zoologichesky Zhurnal. - 2012. - Vol.91. - Iss. 11. - P.1415-1419. - ISSN 0044-5134.
Внешние системы: WoS: 000313139100013;
Реферат: eng: A comparative analysis of quantitative and qualitative characteristics of DNA isolated from various kinds of tissue samples (buccal swabs, ventral scales and tail tips) was carried out. DNA extracted from buccal swabs had 2-3 times smaller concentration, than DNA from other sample types; nevertheless, the DNA content remained high. The extracted DNA has been successfully used for a sequencing of mitochondrial genes, microsatellite, ISSR and AFLP genotyping. While buccal swabs are the least invasive and simple approach, it is a very efficient way for DNA sampling of scaled reptiles.
Ключевые слова: AMPHIBIANS; MULTIPLE PATERNITY; VIPER GLOYDIUS-HALYS;
Издано: 2012
Физ. характеристика: с.1415-1419