| Инд. авторы: | Sanzhaeva U., Vorontsova Y., Glazachev Y., Slepneva I. |
| Заглавие: | Dual effect of nitric oxide on phenoloxidase-mediated melanization |
| Библ. ссылка: | Sanzhaeva U., Vorontsova Y., Glazachev Y., Slepneva I. Dual effect of nitric oxide on phenoloxidase-mediated melanization // Journal of Enzyme Inhibition and Medicinal Chemistry. - 2016. - Vol.31. - Iss. 6. - P.1063-1068. - ISSN 1475-6366. - EISSN 1475-6374. |
| Внешние системы: | DOI: 10.3109/14756366.2015.1088843; PubMed: 26406272; SCOPUS: 2-s2.0-84989313919; WoS: 000385270300025; |
| Реферат: | eng: The study has demonstrated a dual effect of nitric oxide on phenoloxidase (PO)-mediated DOPA oxidation and melanization process. NO generated at low rates proportionally increased in PO-mediated DOPA oxidation. Competitive PO inhibitor, phenylthiourea, resulted in significant inhibition of NO-mediated DOPA oxidation. Further analysis using fluorescent and EPR methods demonstrated that the effect of NO on DOPA oxidation is explained by oxidation of NO to NO2 at the active site of PO followed by oxidation of DOPA by NO2. On the contrary, the bolus addition of NO gas solution resulted in a significant decrease in observed PO activity. Similar dose-dependent effect of NO was observed for the insect’s haemocytes quantified as percentage of melanized cells after treatment with nitric oxide. In conclusion, the results of the study suggest that NO may have a significant regulatory role on melanization process in invertebrates as well as in human and result in protective or damaging effects. © 2015 Informa UK Limited, trading as Taylor & Francis Group.
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| Ключевые слова: | Monophenol Monooxygenase; Melanins; priority journal; oxidation; nonhuman; melanogenesis; Galleria mellonella; fluorescence analysis; enzyme activity; electron spin resonance; concentration response; blood cell; Nitric Oxide; nitric oxide; monophenol monooxygenase; melanin; phenylthiourea; nitric oxide; monophenol monooxygenase; melanin; DOPA; phenoloxidase; nitric oxide; Melanization; Article; |
| Издано: | 2016 |
| Физ. характеристика: | с.1063-1068 |