Инд. авторы:	Kashinskaya E.N., Andree K.B., Simonov E.P., Solovyev M.M.
Заглавие:	DNA extraction protocols may influence biodiversity detected in the intestinal microbiome: a case study from wild Prussian carp, Carassius gibelio
Библ. ссылка:	Kashinskaya E.N., Andree K.B., Simonov E.P., Solovyev M.M. DNA extraction protocols may influence biodiversity detected in the intestinal microbiome: a case study from wild Prussian carp, Carassius gibelio // FEMS Microbiology Ecology. - 2017. - Vol.93. - Iss. 2. - Art.fiw240. - ISSN 0168-6496 . - EISSN 1574-6941.
Внешние системы:	DOI: 10.1093/femsec/fiw240; PubMed: 27915284; SCOPUS: 2-s2.0-85026322376; WoS: 000397431300012;
Реферат:	eng: In this investigation, we examined the influence of different DNA extraction protocols on results obtained for intestinal microbiota of Prussian carp. We showed that significant differences were observed in numbers of reads, OTUs, Shannon index and taxonomic composition between two different DNA extraction protocols for intestine of Prussian carp (Carassius gibelio), and differences were also evident between microbial communities in the intestinal mucosa and intestinal content. Statistical analyses of 25 published articles also revealed a significant relationship between methods of DNA extraction and bacterial diversity in fish intestine of freshwater species. Microbial diversity, community structure, proportions of read numbers derived from each OTU and the total number of OTU's obtained by different DNA extraction protocols could lead to a bias in results obtained in some cases, and therefore researchers should be conservative in conclusions about community structures. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Ключевые слова:	Gastrointestinal Microbiome; Fresh Water; DNA; Carps; Biodiversity; Bacteria; Animals; physiology; microbiology; intestine flora; intestine; Intestines; carp; biodiversity; bacterium; animal; fresh water; DNA; metagenomic sequencing; genetic diversity; fish gut microbiota; extraction of DNA; Carassius gibelio; genetics;
Издано:	2017