Инд. авторы: | Bessmel'tsev V.P., Terent'ev V.S. |
Заглавие: | Calculating the spatial fluorescence distribution of a thick fluorophore layer in a multichannel confocal microscope |
Библ. ссылка: | Bessmel'tsev V.P., Terent'ev V.S. Calculating the spatial fluorescence distribution of a thick fluorophore layer in a multichannel confocal microscope // Journal of Optical Technology. - 2015. - Vol.82. - Iss. 6. - P.374-379. - ISSN 1070-9762. - EISSN 1091-0786. |
Внешние системы: | DOI: 10.1364/JOT.82.000374; SCOPUS: 2-s2.0-84943157151; WoS: 000357489300008; |
Реферат: | eng: The propagation of rays in the optical system of a multichannel confocal microscope is numerically modelled in the Gaussian approximation, making it possible to estimate the shape of the image profile of a focused laser beam in a thick phosphor layer. The proposed method creates the possibility of calculating the signal-to-background- illumination-noise ratio of a confocal system in the case of a large number of parallel channels without using extensive computer resources. The results are compared with experimental measurements made by successively scanning a thick fluorophore layer in depth. (C) 2015 Optical Society of America.
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Издано: | 2015 |
Физ. характеристика: | с.374-379 |