Инд. авторы:	Okotrub K.A., Amstislaysky S.Y., Surovtsev N.V.
Заглавие:	Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing
Библ. ссылка:	Okotrub K.A., Amstislaysky S.Y., Surovtsev N.V. Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing // Archives of biochemistry and biophysics . - 2017. - Vol.635. - P.37-43. - ISSN 0003-9861. - EISSN 1096-0384.
Внешние системы:	DOI: 10.1016/j.abb.2017.10.001; PubMed: 28993092; WoS: 000417663200005;
Реферат:	eng: Although lipid phase transition is believed to be among the major damaging factors in oocytes and pre-implantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between -7 and 0 degrees C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.
Ключевые слова:	POLYMETHYLENE CHAIN; CHILLING SENSITIVITY; DEVELOPMENTAL COMPETENCE; IN-VIVO; FATTY-ACID; FROZEN YEAST-CELLS; H STRETCHING REGION; CRYOPRESERVATION; Raman spectroscopy; Glycerol; Program freezing; Cryopreservation; Lipid phase transition; Preimplantation mouse embryo; INTRACELLULAR ICE FORMATION; OOCYTES;
Издано:	2017
Физ. характеристика:	с.37-43