| Инд. авторы: | Dubovskiy I.M., Grizanova E.V., Whitten M.M.A., Mukherjee K., Greig C., Alikina T., Kabilov M., Vilcinskas A., Glupov V.V., Butt T.M. |
| Заглавие: | Immuno-physiological adaptations confer wax moth Galleria mellonella resistance to Bacillus thuringiensis |
| Библ. ссылка: | Dubovskiy I.M., Grizanova E.V., Whitten M.M.A., Mukherjee K., Greig C., Alikina T., Kabilov M., Vilcinskas A., Glupov V.V., Butt T.M. Immuno-physiological adaptations confer wax moth Galleria mellonella resistance to Bacillus thuringiensis // Virulence. - 2016. - Vol.7. - Iss. 8. - P.860-870. - ISSN 2150-5594. - EISSN 2150-5608. |
| Внешние системы: | DOI: 10.1080/21505594.2016.1164367; PubMed: 27029421; SCOPUS: 2-s2.0-85002002674; WoS: 000390431500003; |
| Реферат: | eng: Microevolutionary mechanisms of resistance to a bacterial pathogen were explored in a population of the Greater wax moth, Galleria mellonella, selected for an 8.8-fold increased resistance against the entomopathogenic bacterium Bacillus thuringiensis (Bt) compared with a non-selected (suspectible) line. Defense strategies of the resistant and susceptible insect lines were compared to uncover mechanisms underpinning resistance, and the possible cost of those survival strategies. In the uninfected state, resistant insects exhibited enhanced basal expression of genes related to regeneration and amelioration of Bt toxin activity in the midgut. In addition, these insects also exhibited elevated activity of genes linked to inflammation/stress management and immune defense in the fat body. Following oral infection with Bt, the expression of these genes was further elevated in the fat body and midgut of both lines and to a greater extent some of them in resistant line than the susceptible line. This gene expression analysis reveals a pattern of resistance mechanisms targeted to sites damaged by Bt with the insect placing greater emphasis on tissue repair as revealed by elevated expression of these genes in both the fat body and midgut epithelium. Unlike the susceptible insects, Bt infection significantly reduced the diversity and richness (abundance) of the gut microbiota in the resistant insects. These observations suggest that the resistant line not only has a more intact midgut but is secreting antimicrobial factors into the gut lumen which not only mitigate Bt activity but also affects the viability of other gut bacteria. Remarkably the resistant line employs multifactorial adaptations for resistance to Bt without any detected negative trade off since the insects exhibited higher fecundity. © 2016 The Author(s). Published with license by Taylor & Francis. © Ivan M. Dubovskiy, Ekaterina V. Grizanova, Miranda M. A. Whitten, Krishnendu Mukherjee, Carolyn Greig, Tatiana Alikina, Marsel Kabilov, Andreas Vilcinskas, Viktor V. Glupov, and Tariq M. Butt.
|
| Ключевые слова: | Larva; Inflammation; Gene Expression Profiling; Gastrointestinal Microbiome; Bacillus thuringiensis; Animals; Adipose Tissue; Adaptation, Physiological; physiology; pathogenicity; moth; microbiology; larva; intestine flora; immunology; genetics; gene expression profiling; Bacillus thuringiensis; animal; adipose tissue; stress; signal transduction; receptor upregulation; Pseudomonas; Note; nonhuman; microbial community; male; inflammation; immunity; humoral immunity; gene expression; Galleria mellonella; female; Enterococcus; Enterobacter; controlled study; Bacillus thuringiensis; animal model; animal experiment; adaptation; monophenol monooxygenase; lysozyme; aminopeptidase; alkaline phosphatase; Moths; microevolution; insect; immune response; experimental evolution; Bt; resistance; |
| Издано: | 2016 |
| Физ. характеристика: | с.860-870 |