| Инд. авторы: | Tkachev S.E., Chicherina G.S., Golovljova I., Belokopytova P.S., Tikunov A.Y., Zadora O.V., Glupov V.V., Tikunova N.V. |
| Заглавие: | New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia |
| Библ. ссылка: | Tkachev S.E., Chicherina G.S., Golovljova I., Belokopytova P.S., Tikunov A.Y., Zadora O.V., Glupov V.V., Tikunova N.V. New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia // Infection Genetics and Evolution. - 2017. - Vol.56. - P.36-43. - ISSN 1567-1348 . - EISSN 1567-7257. |
| Внешние системы: | DOI: 10.1016/j.meegid.2017.10.020; PubMed: 29069610; SCOPUS: 2-s2.0-85032380215; WoS: 000417474500005; |
| Реферат: | eng: Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, is a causative agent of a severe neurological disease. There are three main TBEV subtypes: the European (TBEV-Eu), Far Eastern (TBEV-FE), and Siberian (TBEV-Sib). Currently, three lineages within TBEV-Sib have been recorded. In this study, the genetic and biological characteristics of a new original strain, TBEV-2871, isolated in the Novosibirsk province of Western Siberia, Russia were investigated. The strain has low neuroinvasiveness in mice. Phylogenetic analysis demonstrated that TBEV-2871 belongs to TBEV-Sib, but does not cluster with any of the TBEV-Sib lineages. The TBEV-2871 strain has 88–89% nucleotide sequence identity with the other TBEV-Sib strains, 84–86% nucleotide sequence identity with the TBEV-FE and TBEV-Eu subtypes and is genetically close to the subtype division border. The TBEV-2871 polyprotein sequence includes 43 unique amino acid substitutions, 30 of which are recorded at positions that are conserved among all TBEV subtypes. Strain TBEV-2871 and two similar but not identical isolates found in Kemerovo province, Western Siberia are separated into a new lineage tentatively named Obskaya after the name of Ob riber, in the vicinity of which the TBEV-2871 was first found. A molecular evolution investigation demonstrated that within TBEV-Sib, the Obskaya lineage likely separated 1535 years ago, which is even earlier than the Baltic lineage. © 2017 Elsevier B.V.
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| Ключевые слова: | virus isolation; virus identification; viral genetics; Tick borne encephalitis virus; Russian Federation; priority journal; phylogeny; nucleotide sequence; nucleotide sequence; nonhuman; mouse; virus virulence; female; Article; animal tissue; amino acid substitution; adult; polyprotein; Western Siberia; Tick-borne encephalitis virus; Siberian subtype; Lineages; molecular evolution; virus strain; |
| Издано: | 2017 |
| Физ. характеристика: | с.36-43 |