| Инд. авторы: | Yaroslavtseva O.N., Dubovskiy I.M., Khodyrev V.P., Duisembekov B.A., Kryukov V.Y., Glupov V.V. |
| Заглавие: | Immunological mechanisms of synergy between fungus Metarhizium robertsii and bacteria Bacillus thuringiensis ssp morrisoni on Colorado potato beetle larvae |
| Библ. ссылка: | Yaroslavtseva O.N., Dubovskiy I.M., Khodyrev V.P., Duisembekov B.A., Kryukov V.Y., Glupov V.V. Immunological mechanisms of synergy between fungus Metarhizium robertsii and bacteria Bacillus thuringiensis ssp morrisoni on Colorado potato beetle larvae // Journal of Insect Physiology. - 2017. - Vol.96. - P.14-20. - ISSN 0022-1910. - EISSN 1879-1611. |
| Внешние системы: | DOI: 10.1016/j.jinsphys.2016.10.004; PubMed: 27751890; SCOPUS: 2-s2.0-84992391883; WoS: 000392686100003; |
| Реферат: | eng: The synergistic effect between the entomopathogenic fungus Metarhizium robertsii and a sublethal dose of the bacterium Bacillus thuringiensis ssp. morrisoni var. tenebrionis was studied in terms of immune defense reactions and detoxification system activity of the Colorado potato beetle, Leptinotarsa decemlineata, fourth instar larvae. Bacterial infection led to more rapid germination of fungal conidia on integuments. We found a significant decrease of cellular immunity parameters, including total hemocyte count and encapsulation response, under the influence of bacteria. Phenoloxidase activity in integuments was increased under bacteriosis, mycosis and combined infection compared to controls. However, phenoloxidase activity in the hemolymph was enhanced under bacteriosis alone, and it was decreased under combined infection. Activation of both nonspecific esterases and glutathione-S-transferases in the hemolymph was shown at the first day of mycosis and third day of bacteriosis. However, inhibition of detoxification enzymes was detected under combined infection. The suppression of cellular immunity and detoxification reactions in Colorado potato beetle larvae with a sublethal dose of bacteria is discussed as a reason for synergy between B. thuringiensis and M. robertsii. © 2016 Elsevier Ltd
|
| Ключевые слова: | Metarhizium; Larva; Insect Proteins; Immunity, Innate; Hemolymph; Hemocytes; Beetles; Bacillus thuringiensis; Animals; physiology; microbiology; Metarhizium; metabolism; larva; innate immunity; immunology; hemolymph; growth, development and aging; genetics; enzymology; cytology; chemistry; blood cell; biological pest control; beetle; Bacillus thuringiensis; animal; monophenol monooxygenase; insect protein; Metarhizium; Leptinotarsa decemlineata; Hexapoda; Fungi; Bacteria (microorganisms); Bacillus thuringiensis; United States; Colorado; potato; larva; infectivity; immunity; germination; fungus; enzyme activity; entomopathogenic organism; encapsulation; detoxification; beetle; bacterium; Phenoloxidase; Metarhizium robertsii; Pest Control, Biological; Insect immunity; Glutathione-S-transferase; Esterases; Encapsulation; Bt; Leptinotarsa decemlineata; Monophenol Monooxygenase; |
| Издано: | ENGLAND: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, 2017 |
| Физ. характеристика: | с.14-20 |